Low impact to fixed cell processing aiming transmission electron microscopy

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Low impact to fixed cell processing aiming transmission electron microscopy

In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.

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ژورنال

عنوان ژورنال: Memórias do Instituto Oswaldo Cruz

سال: 2016

ISSN: 1678-8060,0074-0276

DOI: 10.1590/0074-02760150433